Coding

Part:BBa_K1459005:Design

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-07)


PmrA-PmrB(N-term) from Salmonella


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 692


Design Notes

We had to truncate PmrB sequence just before the iron binding tag.


Source

We obtained PmrA-PmrB sequences thanks to kindness of prof. He from the University of Chicago.

References